Phenotypic screening is a powerful tool for identifying novel small molecules as probes and potential therapeutics as well as for identifying genetic regulators of many biological processes. The Broad Institute of MIT and Harvard in Cambridge, Massachusetts have developed Cell Painting, a broadly applicable high-content image-based assay for accessing the valuable biological information about cellular response to treatments such as drug or genetic perturbations that is contained in cell morphology.

The list of compounds were derived from Broad’s Drug Repurposing Hub dataset. Specs was able to source these compounds and assemble the JUMP-Target (306 compounds) and JUMP-MoA (90 compounds in quadruplicate) libraries as a pre-plated set as a 10mM DMSO solution in 384-well plates.